Table 1.
Plasmid Construction Primers
| Construct | Forward Primer* | Reverse Primer* | Plasmid |
|---|---|---|---|
| Vasa1F-GFP | GGGCCCATGTCAGAAGACTGGGGG | ACTAGTATCCCATGATTCATCATCAGCAGCTCC | pSP6 β-globin UTRs |
| Vasa2F-GFP | GGGCCCATGTCAGGAGATAGATCCTGTTATAAC | ACTAGTATCCCATGATTCATCATCAGCAGCTCC | pSP6 β-globin UTRs |
| Vasa4F-GFP | GGGCCCATGTCAGGTGGAAAGTGCTTTAGATGTC | ACTAGTATTCCCATGATTCATCATCAGCAGCTCC | pSP6 β-globin UTRs |
| Vasa6F-GFP | GGGCCCATGTCACTTCTCAGTCAATCTGAATTCC | ACTAGTATCCCATGATTCATCATCAGCAGCTCC | pSP6 β-globin UTRs |
| Vasa3R-GFP | GGGCCCATGTCAGAAGACTGGGGG | ACTAGTGTTTCCGACACGTCCGGTACG | pSP6 β-globin UTRs |
| Vasa4R-GF | GGGCCCATGTCAGAAGACTGGGGG | ACTAGTCCCTGCCTTCTCCTGGACCTCATTG | pSP6 β-globin UTRs |
| Vasa5R-GFP | GGGCCCATGTCAGAAGACTGGGGG | ACTAGTATCTGGATTGGGGCATTCCTTGGC | pSP6 β-globin UTRs |
|
| |||
| Nterm | AAGCTTATGTCAGAAGACTGGGGGAC | CTCGAGTACCATACTTCTGCACAGGTGTTG | pT7-MAT-Flag-2 |
| DEAD | AAGCTTACACCTGTGCAGAAGTATGGTATGC | CTCGAGTCAGGAAGAGGTAGTCGTTGAG | pT7-MAT-Flag-2 |
| Cterm | AAGCTTCTCAACGACTACCTCTTCCTGACG | CTCGAGAACTAGTATCCCATGATTCATCATCAGC | pT7-MAT-Flag-2 |
|
| |||
| EGFP | ACTAGTATGGTGAGCAAGGGCGAGGAG | ACTAGTTACTTGTACAGCTCGTCCATGCCG | pSP6 β-globin UTRs |
|
| |||
| Gus Full-length | ATGGGGCAGAAAGTGTCCGG | TCACTGAAAGAGGAGGTATTTTTTCACC | pGEM-T Easy |
|
| |||
| Gus R146W | ATGGGGCAGAAAGTGTCCGG CGTGGGGCTGGGACTTGGGATGGAAGAAGCTCTTCCACGACTC |
GAGTCGTGGAAGAGCTTCTTCCATCCCAAGTCCCAGCCCCACG TCACTGAAAGAGGAGGTATTTTTTCACC |
pGEM-T Easy |
|
| |||
| GusΔSOCS GusDSOCS |
GGATCCTTATGGGGCAGAAAGTGTCC GGGCCCATGGGGCAGAAAGTGTCC |
GAATTCTCATGAAGGTTCTAGGCCTCCAACATATTTG ACTAGTTCACTGAAAGAGGAGGTATTTTTTCACC |
pGEX-5x-3 pSP6 β-globin UTRs |
*
All primers are shown in the 5′ to 3′ direction