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. Author manuscript; available in PMC: 2012 Jan 21.
Published in final edited form as: J Mol Biol. 2010 Oct 28;405(3):666–678. doi: 10.1016/j.jmb.2010.10.020

Fig. 7. Stopped-flow fluorescence measurements of G:dT misincorporation.

Fig. 7

(A) Addition of GTP to T/2AP-T results in rG:dT misincorporation. (B) Representative time traces of correct and incorrect NTP addition. The 2AP fluorescence increases upon adding the incorrect GTP (200 μM) with kobs = 0.6 s-1 and with the correct ATP (200 μM) with kobs = 100 s-1. (B) GTP concentration dependence of the misincorporation reaction rate constant fit to a hyperbola (Eq. 2) with kpol = 5.2 ± 0.6 s-1 and Kd = 1816 ± 483μM.