Table 2.
Kinetic parameters of single correct NTP addition during T7 RNAP elongation
| rNTP:template base | kpol (s-1) | Kd (μM) | kpol/Kd (μM-1.s-1) | Elongation substrate |
|---|---|---|---|---|
| G:dC | 190 ± 10 | 70 ± 10 | 2.7 | C/2APTn+1 |
| 3'-dG:dC | 45 ± 9 | 25 ±4 | 1.8 | |
| G:dC | 178 ± 15 | 52 ± 17 | 3.4 | C/2AP-NTn+1 |
| A:dT | 145 ± 5 | 71 ± 11 | 2.0 | T/2AP-Tn+1 |
| C:dG | 209 ± 27 | 30 ± 13 | 7.5 | G/2AP-NTn |
| U:dA | 192 ± 20 | 98 ± 22 | 2.0 | A/2AP-NTn+1 |
| 251 ± 23a | 133 ± 20a | 1.9a | ||
| 3'-dU:dA | 235 ± 28 | 185 ± 64 | 1.3 | |
| U:dA | 187 ± 22 | 124 ± 50 | 1.5 | A/2AP-NTn+1 (G-Tn+2) |
a
From the rapid chemical quenched-flow measurements.