. Author manuscript; available in PMC: 2012 Jan 21.

Published in final edited form as: J Mol Biol. 2010 Oct 28;405(3):666–678. doi: 10.1016/j.jmb.2010.10.020

Table 3.

Kinetic parameters for single incorrect NTP addition during T7 RNAP elongation

rNTP:template	k_pol (s^-1)	K_d (μM)	k_pol/K_d (μM^-1.s^-1)	Discrimination^a	Elongation substrate
U:dT			0.001, 0.002^b	2000, 1000	A/2AP-NTn+1^c
			0.0015	1333	A/2AP-NTn+1^c (G-Tn+2)

G:dT	5.2 ± 0.6	1816 ± 483	0.0029	690	T/2AP-Tn+1
	6.9 ± 0.7	3864 ± 754	0.0018	1111	C/2AP-NTn+1^c

Discrimination is calculated from (k_pol/K_d)_correct/ (k_pol/K_d)_incorrect.

From rapid chemical quenched-flow measurements.

From the slow phase of fluorescence change following the fast correct incorporation.