Figure 3.

Selection of the most effective siRNAs targeting TGFBI mRNA and optimization of the concentration of each maximize the inhibition of TGFBIp production by HCECs. Data shown reflect the mean reduction in extracellular TGFBIp production (knockdown) by HCECs after exposure to the optimal concentration of each siRNA compared with the extracellular TGFBIp production by HCECs exposed to the transfection reagent but not to an siRNA (no siRNA control). Experiments were performed three times to obtain a mean knockdown percentage for each siRNA at each of the five concentrations tested.