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Intracellular TGFBIp production by HCECs is reduced after transfection with siRNAs targeting TGFBI mRNA. Western blot analysis of intracellular TGFBIp and GAPDH protein (internal control) isolated from HCECs 24 hours after transfection with the optimal concentration of four different siRNAs targeting TGFBI mRNA. Also shown is intracellular TGFBIp production by HCECs exposed to the transfection reagent but not to an siRNA (no siRNA control) and pure TGFBIp (external standard).
