Immunolocalization of Cx46 and Cx50 in lens fiber cells collected from adult knockout mice. (A) Cx50 localizes along the plasma membrane between lens fiber cells from KOCx46 mice. (B) Cx46 localizes along the membranes between lens fiber cells from KOCx50 mice. (C, D) Lens fiber cells from Cx46, Cx50 double KO mice, shown by phase microscopy, exhibit no immunoreactivity for Cx46 or Cx50. Regions of plasma membrane apposition are indicated by asterisks and DAPI-stained nuclei are seen in all merged images (A, B, D).