Figure 6. Disturbed flow enhanced endothelial ATPSβ membrane translocation and γ/δ T cells binding to the aortic endothelium in vivo.
Following partial ligation of carotid arteries, 8-week-old male ApoE−/− mice (n=6) were fed a high-fat diet and C57BL/6 mice a normal diet for 1 week. The aortic arch, thoracic aorta and left (partially ligated) and right (sham operation) carotid arteries were isolated for en face immunofluorescence analysis. (A) Representative images of ATPSβ (red), Cav-1 (green), and nucleus (blue) from different aortic regions of ApoE−/− mice. (B) Representative images of TCRγ (red) and nucleus (blue) in different regions of aorta of ApoE−/− mice. (C,D) Representative images of TCRγ (green), Mac3 (red, upper panel), and TCRα (red, lower panel) with their nuclei in blue from distinct aortic segments of C57BL/6 and ApoE−/− mice. (E, F) Representative images of ATPSβ (green), TCRγ (red) and nucleus (blue) from different ligated and sham-treated carotid arteries of ApoE−/− and C57BL/6 mice.