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. 2011 Feb 21;11:36. doi: 10.1186/1471-2180-11-36

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Copyright ©2011 Gabbianelli et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zinc-dependent ZinT and ZnuA accumulation in E. coli O157:H7 and S. enterica strains. RG-F116 (zinT::3xFLAG-kan), RG-F117 (znuA::3xFLAG-kan) E. coli O157:H7 strains or PP134 (zinT::3xFLAG-kan) and SA140 (znuA::3xFLAG- kan ilvI::Tn10dTac-cat:: 3xFLAG- kan) S. enterica strains were grown for 16 h in modM9 supplemented or not with various concentrations of ZnSO₄, as indicated. The extracts were analyzed by Western blot. In SA140 strain the chloramphenicol acetyltransferase (CAT) was used as an internal standard.