Table 1.
Transgenic rescue with substitution mutations in RING finger cysteines.
| Transgene | Mutation | Progeny | n |
|---|---|---|---|
| xyEx186 | C681A | 39 ± 6 | 10 |
| xyEx187 | C681A | 51 ± 11 | 7 |
| xyEx188 | C681A | 63 ± 12 | 5 |
| xyEx69 | C684A | 6 ± 2 | 9 |
| xyEx72 | C684A | <1 | 6 |
| xyEx77 | C684A | 0 | 9 |
| xyEx48 | C700A | <1 | 8 |
| xyEx52 | C700A | 1 ± 0.68 | 8 |
| xyEx63 | C700A | <1 | 10 |
| xyEx134 | C703A | 3 ± 1 | 12 |
| xyEx135 | C703A | <1 | 9 |
| xyEx136 | C703A | 3 ± 1 | 10 |
| xyEx73 | C708A | 0 | 5 |
| xyEx78 | C708A | 0 | 5 |
| xyEx80 | C708A | 0 | 5 |
| xyEx74 | C711A | <1 | 9 |
| xyEx81 | C711A | 3 ± 1 | 10 |
| xyEx129 | C711A | 2 ± 0.76 | 6 |
| xyEx130 | C718A | 0 | 9 |
| xyEx131 | C718A | <1 | 9 |
| xyEx132 | C718A | 0 | 9 |
| xyEx85 | C721A | 2 ± 0.60 | 9 |
| xyEx86 | C721A | 5 ± 2 | 10 |
| xyEx87 | C721A | 11 ± 1 | 9 |
| ebEx498 | wild type | 92 ± 16 | 11 |
| xyEx175 | wild type | 123 ± 15 | 10 |
| xyEx177 | wild type | 115 ± 17 | 11 |
| N2 (wild type) | - | 185 ± 5 | 10 |
| spe-42(tn1231) | - | 0 | 24 |
| spe-42(tm2421) | - | 0 | 15 |
Live progeny were counted for three independently-derived transgenes for each amino acid substitution. All transgenes are in a spe-42(tn1231 or tm2421) null genetic background. N2 and spe-42 worms listed at the bottom do not carry transgenes. All counts were conducted until the first day each worm produce no live progeny. Counts shown are ±SEM and were rounded to the nearest whole number with the exception of SEM values less than one. spe-42(tn1231) data are from [13].