Table 1.

Oligonucleotide Primers Used to Generate cDNAs Encoding Myocilin, SPARC, and Hevin cDNAS Used in Yeast Two-Hybrid Assays and to Express Recombinant Proteins in HEK-293T Cells

Primers	5′→3′ Sequence	Restriction Site
Primers for Yeast Two-Hybrid Assays
1	AATAGAATTCACAGCTCAGCTCAGGAAGGCC	Eco-RI
2	TTATGGATCCTCACATCTTGGAGAGCTTGATGTC	Bam-HI
3	TTTTGAATTCTCTGGCTATCTCAGGAGTGG	Eco-RI
4	GGTGCTCGAGTCAAAACAAGAGATTTTCATC	Xho-I
5	AAATGAATTCTAAAATCTATTCCTACTTGTACGG	Eco-RI
6	TGTTCTCGAGTTAGATCACAAAGATCCTTGTCG	Xho-I
7	CAACGGATCCTAATCCCCCCTTGCCTGG	Bam-HI
Primers for Expression of Recombinant Proteins in HEK-293T Cells
8	GCCCTCTAGATGAGGGCCTGGATCTTCTTTCTCC	Xba-I
9	GCTCGGATCCAGGATCACAAGATCCTTGTCG	Bam-HI
10	GTGCAGGCAAGGGGGGATTGCCAAGGCCCTCCCGGC	—
11	GCCGGGAGGGCCTTGGCAATCCCCCCTTGCCTGGAC	—

Sequences in bold italic type indicate restriction sites introduced in primers to facilitate subcloning of PCR products.