Table 1.
Primers | 5′→3′ Sequence | Restriction Site |
---|---|---|
Primers for Yeast Two-Hybrid Assays | ||
1 | AATAGAATTCACAGCTCAGCTCAGGAAGGCC | Eco-RI |
2 | TTATGGATCCTCACATCTTGGAGAGCTTGATGTC | Bam-HI |
3 | TTTTGAATTCTCTGGCTATCTCAGGAGTGG | Eco-RI |
4 | GGTGCTCGAGTCAAAACAAGAGATTTTCATC | Xho-I |
5 | AAATGAATTCTAAAATCTATTCCTACTTGTACGG | Eco-RI |
6 | TGTTCTCGAGTTAGATCACAAAGATCCTTGTCG | Xho-I |
7 | CAACGGATCCTAATCCCCCCTTGCCTGG | Bam-HI |
Primers for Expression of Recombinant Proteins in HEK-293T Cells | ||
8 | GCCCTCTAGATGAGGGCCTGGATCTTCTTTCTCC | Xba-I |
9 | GCTCGGATCCAGGATCACAAGATCCTTGTCG | Bam-HI |
10 | GTGCAGGCAAGGGGGGATTGCCAAGGCCCTCCCGGC | — |
11 | GCCGGGAGGGCCTTGGCAATCCCCCCTTGCCTGGAC | — |
Sequences in bold italic type indicate restriction sites introduced in primers to facilitate subcloning of PCR products.