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. 2011 Mar 10;6(3):e17682. doi: 10.1371/journal.pone.0017682

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Ciavarra et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Confocal microscopy analysis for BrdU incorporation in ctrl, mgRb:Rb^−/− and mgRb:Rb^−/−:p130^−/− myotubes at DM-2. Myoblasts were differentiated for 1 day, then exposed to 20 µM BrdU for an additional 16 hr in the presence of growth medium (GM) and immuno-stained for MHC (red) and BrdU (green). Arrowheads label BrdU positive nuclei within myotubes. (B) MHC (red) and TUNEL (green) staining at DM-2. Arrowheads indicate TUNEL positive nuclei, which are invariably located outside myotubes. (C) Mitotracker® (red) staining at DM-2. Arrowheads point to large Mitotracker®-positive perinuclear aggregates.