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. 2011 Mar 10;6(3):e17682. doi: 10.1371/journal.pone.0017682

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Ciavarra et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunostaining for MHC (green) in Ad.EV and Ad.cre transduced Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− myoblast cultures at DM-2. Nuclei were counterstained with DAPI. (B) Quantification of percent multinucleated myotubes relative to total number of MHC-positive cells (myocytes plus myotubes) in Ad.EV and Ad.cre transduced Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− myoblasts at DM-2 under normoxia. Numbers within bars indicate % for the respective samples.