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. 2011 Mar 10;6(3):e17682. doi: 10.1371/journal.pone.0017682

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Ciavarra et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunostaining for MHC (green) of Ad.EV (a,c,e,g) or Ad.cre (b,d,f,h) transduced Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− cultures at DM-5 in hypoxia. Inlets, DAPI staining for nuclei. (B) Quantification of myotube formation in Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− cultures transduced with Ad.EV or Ad.cre and induced to differentiate 48 hr later for 5 days. Counts represent the average number of myotubes at DM-5 of 6 representative fields (n = 4); error bars represent s.d. *-p<0.05 and **-p<0.07. (C) Quantification of percent multinucleated myotubes relative to total number of MHC-positive cells in Ad.EV or Ad.cre transduced Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− cultures at DM-5 under hypoxia. Numbers within bars indicate % for respective samples. (D) Quantification of myotube formation in Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− cultures transduced with Ad.EV or Ad.cre and induced to differentiate in hypoxia. Counts were conducted at DM-2 and DM-6. Error bars represent s.d.