Table 2. Y2H-derived PPI data confirmed by alternative and supplementary experimental methods.

Interactor A	Interactor B	Y2H	GST/His-Tag pull-down	Biochemical activation	Motif mapping	Surface plasmon resonance
Imp3	Mpp10	✓ [46]	✓ [46]		✓ [46]
Imp4	Mpp10	✓ [46]	✓ [46]		✓ [46]
Esf2	Dbp8	✓ [43]	✓ [43]	✓ [43]
Pfa1	Prp43	✓ [48], [89]	✓ [89]	✓ [89]	✓ [89]
t-Utp8	t-Utp9	✓ [39], [44]	✓ [44]	✓ [44]	✓ [44]
Utp6	Utp21	✓ [38]			✓ [38]	✓ [38]
Utp6	Utp18	✓ [38]			✓ [38]
Utp25	Utp3	✓ [41], [51]	✓ [41]		✓ [41], [51]

Many PPIs from the LC data have alternative forms of supporting evidence from experiments that test for binary interactions, including pull-downs, activation of enzymatic activities, motif mapping by truncations and surface plasmon resonance. This list of protein-protein interactions identified by Y2H and validated by independent methods is not exhaustive.