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. 2011 Mar 10;6(3):e17809. doi: 10.1371/journal.pone.0017809

Figure 4. Growth curves and metabolic rate of 293TetER, EREV8, and ERKV cells with or without Dox treatment.

Figure 4

(A) Cell growth rates of 293TetER, EREV8, and ERKV were determined by seeding triplicate wells (20,000 cells/well) in a 24-well tissue culture plate for each time point. In parallel, duplicate plates were prepared, and 50 ng/ml Dox was added to each sample 24 h after cell seeding. At the specified times, cells were counted using the trypan blue-exclusion method. Error bars denote standard deviations of triplicate wells. Four independent experiments were performed, one set of representative results is shown. ST, starting numbers. (B) Cell metabolic activity was measured using the WST-1 assay. Cell preparations and Dox treatment were identical to the procedure described in (A). Three independent experiments were performed, and one set of representative results is shown. (C) Disrupted morphologic changes were observed in 120 h Dox-treated EREV8 and ERKV cells, but not in 293TetER cells that exhibited cellular senescence.