Figure 1. The effect of initial cell population and inductive signals on cell proliferation.

(A) Protocols adopted to drive the differentiation of CD34⁺, CD34⁺KDR⁻ and CD34⁻ cells isolated from EBs at day 10 into the SMC lineage. (B) Flow cytometric analysis of hES-derived cells: CD34⁻ (B.1), CD34⁺ (B.2) and CD34⁺KDR⁻ (B.3) cells (in this last case isolated from the CD34⁺ cell population). The results show that CD34⁻ cells do not express CD34⁺ marker (B.1), CD34⁺ cells are formed by CD34⁺KDR⁻ and CD34⁺KDR⁺ cells (B.2), and CD34⁺KDR⁻ do not express the KDR marker (B.3). Percent of positive cells (dash plot) were calculated based in the isotype controls (grey plot). (C) Time-course proliferation of CD34⁺ (C.1), CD34⁺KDR⁻ (C.2) and CD34⁻ cells (C.3).