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. 2011 Mar 10;6(3):e17771. doi: 10.1371/journal.pone.0017771

Figure 4. Contractility of hESC-derived cells.

Figure 4

(A) Concentration of intracellular Ca2+ in FURA-2-loaded cultured hESC-derived cells in response to several agonists (100 µM histamine, 10−7 M bradykinin, 10−5 M angiotensin II or 50 mM KCl). hVSMCs and HUVECs were used as positive and negative controls, respectively. Traces are representative of 4 independent experiments for each condition. (B) Contractility of hESC-derived cells when exposed to the effects of carbachol (10−5 M in DMEM medium, for 30 min) or atropine (10−4 M, 1 h) plus carbachol (10−5 M for 30 min). hVSMCs were used as controls. Results are Mean ± SEM (n = 3); # denotes statistical significance (P<0.05) in the same experimental group.