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. 2011 Mar 10;6(3):e17771. doi: 10.1371/journal.pone.0017771

Figure 6. Characterization of hESC-derived SMPCs encapsulated in 3D fibrin gel scaffolds.

Figure 6

SMPCs were characterized after being encapsulated in fibrin gels for 3 days. (A) SMC gene expression on CD34+RA (A.1) and CD34+PDGF (A.2) cells, as assessed by qRT-PCR. Gene expression of hESC-derived SMPCs was normalized by gene expression of hVSMCs under the same culture conditions. Results are Mean ± SEM (n = 4). (B) Comparison of extracellular matrix and adhesion molecules-related gene expression in CD34+PDGFBB (B.1) or CD34+RA cells (B.2) with hVSMCs cultured in 2D (tissue culture polystyrene) or cultured in 3D fibrin gels. Gene expression was evaluated using a RT2 Profiler™ PCR array. (C) Normalized extracellular matrix and soluble factor gene expression of SMPCs relatively to hVSMCs, both cultured in 3D or 2D systems.