Table 4.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| Rhizobium tropici strains | ||
| CIAT899 | wild type; Acid tolerant, Nalr | (Martínez-Romero et al., 1991) |
| 899-olsCΔ1 | CIAT899 carrying a 211 bp nonpolar deletion in olsC | (Rojas-Jiménez et al., 2005) |
| MAV04 | CIAT899 carrying a deletion in olsE | This work |
| MAV05 | CIAT899 carrying deletions in olsC and olsE | This work |
| Sinorhizobium meliloti strains | ||
| CS111 | pssA-deficient mutant of wild type 1021 | (Sohlenkamp et al., 2004) |
| Burkholderia cenocepacia strains | ||
| J2315 | wild type | (Holden et al., 2009) |
| Escherichia coli strains | ||
| DH5α | recA1, Φ80 lacZΔM15; cloning strain | (Hanahan, 1983) |
| S17-1 | thi pro recA hsdR− hsdM+ RP4 integrated in the chromosome, 2-Tc::Mu, Km::Tn7(Tpr/Smr) | (Simon et al., 1983) |
| Plasmids | ||
| pET17b | Expression vector, Cbr | (Studier, 1991) |
| pET9a | Expression vector, Kanr | (Studier, 1991) |
| pRK404 | Broad-host-range vector, tetracycline resistant | (Ditta et al., 1985) |
| pBBR1MCS | Broad-host-range plasmid, chloramphenicol-resistant | (Kovach et al., 1994) |
| pUC18 | Cloning vector, ampicillin-resistant | (Yanisch-Perron et al., 1985) |
| pRK2013 | Helper plasmid; Kmr | (Ditta et al., 1985) |
| pVK102 | cosmid vector | (Vargas et al., 1990) |
| pK18mobsacB | conjugative suicide vector, kanamycin resistant | (Schäfer et al., 1994) |
| pNG23 | olsB of Burkholderia cenocepacia cloned in pET17b | This work |
| pNG25 | olsB of B. cenocepacia subcloned as a BglII/HindIII fragment from pNG23 into BamHI/HindIII-digested pBBR1MCS | This work |
| pCCS98 | olsC of R. tropici in pET9a | This work |
| pCos94 | pVK102 derivative containing the olsE gene | This work |
| pEMAV01 | 1 kb fragment upstream of olsE, cloned as SmaI/BamHI fragment in pUC18 | This work |
| pEMAV02 | 1 kb fragment downstream of olsE, cloned as BamHI/HindIII fragment in pUC18 | This work |
| pEMAV03 | 1 kb upstream and 1kb downstream sequences flanking olsE, cloned into pUC18 | This work |
| pPMAV04 | Suicide vector for construction of mutant MAV04 and MAV05 | This work |
| pURMAV03 | olsE-containing 3.5 kb fragment of pCos94 cloned as PstI/PstI fragment in pUC18 | This work |
| pERMAV04 | olsE-containing 3.5 kb fragment of pCos94 cloned as PstI/PstI fragment in pRK404 | This work |
| pERMAV06 | pET9a cloned as a BamHI fragment into pRK404 | This work |
| pEMAV07 | ORF1 in pET9a | This work |
| pEMAV08 | ORF2 in pET9a | This work |
| pEMAV09 | olsE in pET9a | This work |
| pERMAV11 | pEMAV07 cloned as a BamHI fragment into pRK404 | This work |
| pERMAV12 | pEMAV08 cloned as a BamHI-fragment into pRK404 | This work |
| pERMAV13 | pEMAV09 cloned as a BamHI-fragment into pRK404 | This work |
| pERMAV15 | pCCS98 cloned as a BamHI fragment into pRK404 | This work |
| pEMAV16 | olsC cloned as a BamHI/BglII fragment into BamHI-digested pEMAV09 | This work |
| pERMAV17 | pEMAV16 cloned as a BamHI fragment into pRK404 | This work |