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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Cancer Ther. 2011 Jan 20;10(3):550–557. doi: 10.1158/1535-7163.MCT-10-0571

Figure 3.

Figure 3

Effect of siRNA knockdown of EGFR and/or P63 in TNBC cells. CRL2335 TNBC cells were transfected with both EGFR siRNA and P63 siRNA and MDA-MB-468 cells were transfected with EGFR siRNA alone or random siRNA. After 48h of transfection, cells were treated with or without TRAIL for additional 24h. Whole cell extracts were prepared and analyzed for EGFR, P63 by Western blotting (A, C). Equal protein loading was compared with that of GAPDH. (B, D) Under similar conditions cell viability was assessed using trypan blue exclusion method.