Table 1.
The selected SNPs (position/function), designed primers and amplicon sizes.
| Gene | SNP | SNP position* | Function | Primers Forward/Reverse |
Amplicon size |
|---|---|---|---|---|---|
| BMP-2 | rs1005464 | intron 2 c.347-2744 G > A |
intron | 5'-TGAGCGTATATTCCCTAACC-3' 5'-TAACCTCCCAAAAAATTAAATGAC-3' |
378 bp |
| rs235768 | exon 3 c.570 A > T |
missense mutation (p.R190S) |
5'-GCAGAGCTTCAGGTTTTCCG-3' 5'-TGTTTCTCCTCCAAGTGGGC-3' |
269 bp | |
| rs235764 | intron 2 c.346+3126 G > A |
intron | 5'-ACTGACATTTTCCGTTCCACCT-3' 5'-TAACAGACAACTGATCAAGGAG-3' |
305 bp | |
| BMP-7 | rs4811822 | intron 2 c.612-1290 C > T |
intron | 5'-CCCAGGGCAACAACAGTCTC-3' 5'-CCTGGGCACACAACTTGACC-3' |
260 bp |
| rs1475000 | intron 2 c.611+10288 G > A |
intron | 5'-TGCAGATGCTGGGTCCTTAA-3' 5'-CGGGTCAGATGCCCATGAAG-3' |
281 bp | |
| rs186659 | intron 1 c.419-2863 G > A |
intron | 5'-CTGCAGGGCCTCATACACTA-3' 5'-GAGAACAGCTTCCAGGGTGA-3' |
292 bp | |
| NOG | rs1442828 | intragenic g.13328730 A > G |
intragenic | 5'-TCCTCTTCGGTCATCCAGTG-3' 5'-TGGTGGAAACCTTGCCATTC-3' |
199 bp |
| rs1372857 | intragenic g.13334320 A > G |
intragenic | 5'-CTGGGAGGGTTCTTGATTGG-3' 5'-ACATGTGAAATGCAGGGCAG-3' |
170 bp | |
| rs9915822 | intragenic g.13320012 G > T |
intragenic | 5'-TTAGGCGTCACCCACAGTTG-3' 5'-TGGGCAAGGTAAATGGAAGC-3' |
190 bp | |
| SMAD6 | rs2053423 | intron 3 c.13320013 C > T |
intron | 5'-CATGGCTTGGATGCTTGGTGT-3' 5'-TTCCCAGTCCAAATCAGGGT-3' |
398 bp |
| rs2119261 | intron 3 c.952+3144 C > T |
intron | 5'-GCCACTACTGGACAAACCTT-3' 5'-TCCAACAACTACTCGGCAGA-3' |
414 bp | |
| rs3934908 | intron 3 c.953-11868 C > T |
intron | 5'-GAATTGGATGGAGACACGTACC-3' 5'-GATCTGGAATGCTTCCTGAG-3' |
542 bp | |
(* SNP position according to NCBI SNP: Geneview)