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. Author manuscript; available in PMC: 2011 Sep 14.
Published in final edited form as: Biochemistry. 2010 Sep 14;49(36):7854–7866. doi: 10.1021/bi1008408

Figure 2.

Figure 2

A) Hemolytic activities of Fl-E5-TAT at pH 7.0 (diamonds) and 4.0 (triangles) as a function of peptide concentration and for 1.25% RBC suspensions. The data fit the equations y = 27.2× at pH 7 and y= 4.9× at pH 4.0 with R2 of 0.90 and 0.98, respectively. The HD50 of the peptide are 1.8 and 10.2 µM at pH 4.0 and 7.0, respectively. B) Bright field image of intact RBCs in the absence of peptide. C) Bright field image of ghosts obtained by E5-TAT mediated hemolysis of RBCs at pH 4.5 and at a concentration below the HD50 of the peptide at this pH. D) Bright field image of RBCs lysed by E5-TAT at pH 7.0 at a concentration twice its HD50. Ghosts fuse to one another and their membranes collapse rapidly after addition of the peptide.