Fig. 3.

Growth of strains after protein depletion. Strains carrying ID tags fused to GyrA, KasA, and RpoB were grown to midlog phase and preincubated with ATc for 12 h to allow protein degradation to proceed. Strains harboring ID tag fusions to Alr, DFHR, and InhA were not preincubated. To measure growth, strains were diluted 1:100 into fresh medium with or without ATc, and samples were removed at indicated time points for cfu determination. Insets show the amount of remaining protein at indicated time points as determined by Western blotting using anti–c-myc antiserum. A strain carrying the HIV-2 protease gene but no ID tag grew normally in the presence of ATc (Fig. S4).