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. 2011 Feb 22;108(10):3918–3923. doi: 10.1073/pnas.1017425108

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Fig. 1. — Comparison of the domain organization of E. coli IF2 and mammalian IF2_mt, and cryo-EM structure of the complex. (A) Domain alignment of E. coli IF2 and bovine IF2_mt. Note that the mature IF2_mt (i.e., after deletion of the import sequence) starts from aa residue 78. The 37-aa insertion in IF2_mt is highlighted in red. (B) The cryo-EM map of the E. coli 70S ribosome (30S subunit, yellow; 50S subunit, blue) in complex with IF2_mt (red), initiator tRNA (green) at the P/I position, and an additional tRNA density (brown, visible in panels C and D) at the ribosomal E site. (C and D) The 30S and 50S subunit portions of the map are shown from their interface sides to reveal the overall binding positions of IF2_mt and tRNAs. Landmarks of the 30S subunit: h, head; sh, shoulder; b, body; and pt, platform. Landmarks of the 50S subunit: L1, L1 protein protuberance; CP, central protuberance; and Sb, L7/L12 stalk base. For stereo viewing, see Fig. S2.

Inline graphic — Comparison of the domain organization of E. coli IF2 and mammalian IF2_mt, and cryo-EM structure of the complex. (A) Domain alignment of E. coli IF2 and bovine IF2_mt. Note that the mature IF2_mt (i.e., after deletion of the import sequence) starts from aa residue 78. The 37-aa insertion in IF2_mt is highlighted in red. (B) The cryo-EM map of the E. coli 70S ribosome (30S subunit, yellow; 50S subunit, blue) in complex with IF2_mt (red), initiator tRNA (green) at the P/I position, and an additional tRNA density (brown, visible in panels C and D) at the ribosomal E site. (C and D) The 30S and 50S subunit portions of the map are shown from their interface sides to reveal the overall binding positions of IF2_mt and tRNAs. Landmarks of the 30S subunit: h, head; sh, shoulder; b, body; and pt, platform. Landmarks of the 50S subunit: L1, L1 protein protuberance; CP, central protuberance; and Sb, L7/L12 stalk base. For stereo viewing, see Fig. S2.