Fig. 2.

Notch reporter analysis in the ethanol-exposed embryonic cortex. Flow cytometric analysis of the Notch signaling reporter activity in vivo. Cp-EGFP reporter transgenic mouse embryos were subjected to ethanol or PBS (control), and the numbers of EGFP⁺ and Nestin⁺ cortical cells were analyzed. The red numbers indicate the percentage of EGFP⁺ cells (in the range shown by two-headed arrows) in the total Nestin⁺ cells (within the boxed area). The percentage is increased by mFAE. Mean increase compared with the control is 30 ± 4.8% (SEM) (n = 16, P = 0.008 by t test).