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. 2011 Feb 22;108(10):3994–3999. doi: 10.1073/pnas.1014406108

Fig. 1.

Fig. 1.

Reduction of TORC1 activity suppresses the temperature sensitivity of ipl1-2. (A) Cultures of WT (KT1113), YPI1-GFP tco89-71 (KT2998), ipl1-2 (KT1829), ipl1-2 YPI1-GFP (KT3007), ipl1-2 tco89-71 (KT3003), and ipl1-2 YPI1-GFP tco89-71 (KT3000) strains were serially diluted onto medium containing 1% yeast extract, 2% peptone, 2% glucose (YPD) and imaged after 40 h at the indicated temperatures. (B) Cultures of WT (KT1113), ipl1-2 (KT1829), ipl1-2 glc8Δ (KT3005), ipl1-2 tco89-71 (KT3003), ipl1-2 glc8Δ tco89-71 (KT3004), ipl1-2 YPI1-GFP tco89-71 (KT3007), ipl1-2 tor1Δ (KT3010), and ipl1-2 tor1Δ YPI1-GFP (KT3011) strains were treated as in A. (C) Cultures of WT (KT1113), ipl1-2 (KT1829), and ipl1-2 YPI1-GFP (KT3007) strains were serially diluted onto YPD medium and YPD containing 2.5 mM caffeine and treated as in A. (D) Diluted cultures of ipl1-2 (KT1829) were plated onto YPD medium, overlaid with a 7.5-mm paper disk containing 0.1 nM rapamycin or 2.5 μM caffeine, and incubated at the designated temperatures for 48 h (caffeine) or 72 h (rapamycin) before imaging. (Scale bar: 10 mm.) (Insets) Microscopic images were acquired from the designated locations on the plates after 72 h. (Scale bar: 200 μm.)