Fig. 4.
Yield of soluble protein and kinetic parameters of homodimers of 11β-HSD1 in the bacterial expression system. (A) Yield of soluble, purified 11β-HSD1 protein. Values are given in mg of protein purified per L of LB broth. Bars represent SEM (n = 3). (B) Turnover rates (kcat) for the conversion of cortisol to cortisone. The decrease observed for the R137C mutant is statistically significant (P < 0.05, ANOVA). No data are available for the K187N mutant, because no soluble protein was produced. (C) Km values for cortisol for WT and mutant proteins. The increase observed for the R137C mutant compared with WT is not statistically significant. No data are presented for the K187N mutant, because no soluble protein was produced. Bars represent SEM (n = 3).