Table 1.

Composition and geometric mean of mouse IgG of anti-B. pertussis Tohama I LPS induced by conjugates of B. pertussis and B. bronchiseptica core fractions bound to BSA

Conj. no.	OS in conjugate	Conj. Mm, kDa	Saccharide Mm, kDa	S/P ratio	No. of chains per BSA	No. of band A trisaccharide repeats	Terminal saccharide	IgG (25–75%), EU
1	B.p. Tohama I	94	19	0.3	8	1	GlcNAc	4.2 (2.0–7.4)
2	B.p. Tax113	132	57	0.9	25	1	GlcNAc	1.2 (0.5–1.9)
3	B.p. Tax113	115	40	0.6	17	1	GlcNAc	5.6 (3.2–9.0)
4	B.p. Tax113	100	25	0.4	11	1	GlcNAc	3.3 (1.6–6.5)
5	B.b. RB50 core	97	21	0.3	9	1	GlcNAc	4.6 (4.3–7.2)
6	B.b. RBwbmΔ	120	45	0.7	19	1	GlcNAc	2.6 (2.1–4.5)
7	B.b. RBwbmΔ	102	27	0.4	11	1	GlcNAc	2.8 (0.9–7.1)
8	B.b. RBB1a	121	46	0.7	20	1	GlcNAc/GalNAc	0.4 (0.1–0.9)
9	B.b. RBB1a	104	29	0.4	12	1	GlcNAc/GalNAc	8.7 (5.3–14.3)
10	B.b. RBA2b, F4	98	25	0.4	8	1	GlcNAc	5.3 (2.1–16.3)
11	B.b. RBA2b, F3	109	34	0.5	9	2	GlcNAc	0.3 (0.1–4.1)
12	B.b. RBA2b, F2	107	32	0.5	11	3	GlcNAc	0.1 (0.1–0.3)
13	B.b. RBA2b, F1	111	36	0.4	9	4–5	GlcNAc	0.3 (0.1–2.5)
—	PBS	—	—	—	—	—	—	<0.01

Mice (10 per group) were injected with 2.5 μg of saccharide as a conjugate per mouse, s.c., three times 2 weeks apart and bled 1 week after last injection. All conjugates induced statistically higher antibody levels than PBS group. Conjugate 10 vs. 11, 12, and 13, P < 0.005; conjugate 9 vs. 8, P = 0.03. Conj., conjugate; B.p., B. pertussis; B.b., B. bronchiseptica; Mm, molecular mass; S/P, saccharide/protein; –, not applied.