Analysis of core transcription machinery in hepatoblasts and hepatocytes. (A) Immunoblot of purified hepatoblasts (Blast) and hepatocytes (Cyte) for TBP, TFIIB, TFIIE, TFIIH, RNA polymerase II, and actin. (B) Immunoblot for canonical TAFs. (C) Fluorescence-based quantitative immunoblot of hepatoblasts and hepatocytes for TBP, TAF4, and actin spanning two orders of magnitude.