Figure 5.

Chromatin at endogenous DNA replication origins is more accessible during G₁ than in S phase. HaCaT cells were synchronized by serum deprivation and verified by BrdU incorporation as in Figure 1A. qPCR was performed on DNase I-treated chromatin samples from the indicated time points. Results were normalized against input chromatin from each time point that was not treated with DNase I to account for increases in DNA levels during S phase. Assays were performed in triplicate to generate error bars.