Table 2.
Quantification of effects of HBO1, HDAC11 and Set8-HBD on chromatin unfolding
| Proteins expressed | Decondensed (open) chromatin | Condensed (closed) chromatin | Indeterminate |
| LacI-Cdt1 + pcDNA3 | 62% (±5%) | 32% (±2%) | 6% |
| LacI-Cdt1 + Flag-HDAC1 | 58% (±3%) | 35% (±2%) | 7% |
| LacI-Cdt1 + Flag-HDAC11 | 11% (±2%) | 69% (±3%) | 20% |
| LacI-Cdt1 + Flag-HBO1-wt | 65% (±3%) | 35% (±2%) | 0% |
| LacI-Cdt1 + Flag-HBO1G485A | 41% (±3%) | 58% (±3%) | 1% |
| LacI-VP16 + pcDNA3 | 64% (±3%) | 33% (±2%) | 3% |
| LacI-VP16 + Flag-HDAC1 | 60% (±3%) | 35% (±2%) | 5% |
| LacI-VP16 + Flag-HDAC11 | 60% (±3%) | 34% (±2%) | 6% |
| LacI-VP16 + Flag-HBO1-wt | 62% (±3%) | 32% (±2%) | 6% |
| LacI-VP16 + Flag- HBO1G485A | 61% (±3%) | 29% (±2%) | 10% |
| LacI-Cdt1 + Flag-HDAC1 | 67% (±2%) | 30% (±3%) | 3% |
| LacI-Cdt1 + Flag-Set8-HBD | 41% (±2%) | 51% (±2%) | 8% |
| LacI-Cdt1 + GST | 62% (±2%) | 29% (±3%) | 9% |
In each case, at least 150 transfected cells were scored. The results depicted are representative of three experiments performed for each condition with similar outcomes (used for errors, SD; Indeterminate errors were all less than 2%).