Figure 1.

FGF causes an immediate, transient delay in mitotic entry in RCS cells. (A) FACScan™ analysis of RCS cells treated with FGF1 and harvested at the indicated periods of time. Mitotic cells were stained with anti-phospho-histone H3(S10) antibodies. Numbers on the Y-axis indicate percentage of total cells in G₁, S, G₂ and M phases. (B) RCS cells were treated with either Nocodazole alone or together with FGF1. Only changes in the levels of G₂ and M phases are indicated. The data are representatives of several independent experiments with the same results.