Figure 9.

(A) Untransfected HeLa cells were fixed with acetone/methanol and subjected to immunofluorescence staining with anti-CCDC69 (green), anti-Plk1 (red), and DAPI (blue). (B) HeLa cells were transfected with control (siCont) or CCDC69 (siCCDC69) siRNAs. 72 h after transfection, the transfected cells were fixed with acetone/methanol and subjected to immunofluorescence staining with anti-CCDC69, anti-Plk1, and DAPI. Bar, 5µm. (C) Autoradiogram following SDS-PAGE of samples from in vitro kinase assays carried out to measure the activity of Plk1 and aurora B towards GST-CCDC69. (D) A Coomassie stained gel of GST-CCDC69 that was used in C. Bar, 5µm.