Table 1.

Primers used for pfcrt sequencing and real-time PCR quantification

Exon 1-2
Primary
E1/2-F	CGACATTCCGATATATTATATTTTTAGAC
E1/2-R	TATATGTGTAATGTTTTATATTGG
Nested
E1/2-NF	CCGTTAATAATAAATACACGCAG
E1/2-NR	AATGTTTTATATTGGTAGGTGG
Exon 3-8
Primary
E3/8-F	CCACCTACCAATATAAAACATTAC
E3/8-R	GTTAAAATATATATAAATGTCTC
Nested
E3/8-NF	TATATATATATGTATGTATGTTG
E3/8-NR	AATGTCTCTTATAATTTTGAAATT
Exon 9-13
Primary
E9/13-F	CTTATAATAAAATTTCAAAATTATAAGAGAC
E9/13-R	GAGATCTCTATACCTTCAACATTATTCC
Nested
E9/13-NF	GAGACATTTATATATATTTTAAC
E9/13-NR	CCTTATAAAGTGTAATGCG
pfcrt RT-PCR
pfcrt-F	CTTTATTTGTATGATTATGTTCTTTATTGTTTATTCCTTATTTGGA
pfcrt-R	AACAGGCATCTAACATGGATATAGCAAA
pfcrt-probe	TCGGTGTCGTTCTTTTG
pfmdr RT-PCR
pfmdr-F	GCATTCGGTTTTTGGTATGGTACAA
pfmdr-R	CCTAATAAAATGGATATAACTGAGGCACCA
pfmdr-probe	ACGAATCAATACCCCAATAAT
EF-1a RT-PCR
EF-1a-F	GGCAGAAAGAGAAAGAGGTATTACCA
EF-1a-R	CCTGGTGCATCAATGACAGTAAAGA
EF-1a-probe	TTATGGAAATTTGAAACCCC