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. 2011 Feb 15;11:8. doi: 10.1186/1471-213X-11-8

Table 2.

Description of RT-PCR or PCR primers for examined genes

Gene name Gene symbol Primer sequences 5'-3' Amplicon size (bp) Annealing/Melting temp. (°C) Accession number
Cyclin B1 CCNB1 F: ACC TGG CAA AGA ATG TGG TC
R: GCT GTG CTA GAG TGC TGA TCT TAG
108 60/80 NM_001045872
Cyclin Dependant kinase 1 CDK1 F: GAT CCT GCC AAA CGA ATT TCT GGC
R: TCT GCT CTT GAC ACA ACA CAG GGA
121 60/78 NM_174016
Oocyte maturation factor MOS MOS F: CAA AGC ATT GTG CAC TTG GAC CTC
R: TGG GTG TAA CAG GCT CTC CTT TGA
190 60/89 XM_590874
Actin beta ACTB F: CGCCATGGATGATGATATTG
R: GGTCATCTTCTCACGGTTGG
363 60/N/A NM_173979