Skip to main content
. 2010 Jul 14;35(10):2009–2020. doi: 10.1038/npp.2010.85

Figure 2.

Figure 2

Top panel: Dnmt1 is localized to primary neurons. Confocal laser scanning microscopy of primary cortical cultures (DIV 10) fixed with 4% paraformaldeyhde and stained with rabbit polyclonal antibody DNMT1 antibody and mouse monocloncal neuronal nuclear marker antibody (NeuN) DNMT1 appears in red (Cy5 flurophore) and NeuN appears in green (Cy2 flurophore). Percentage of colocalization was 88% ±3 for DNMT1/NeuN. ((a) DNMT1 Sigma D4567, 1: 250; (b) NeuN; Chemicon MAB677, 1: 500, (c) is merge). Bottom panel: DNMT1 colocalizes with double-stranded DNA; (d) is as above; (e) is performed by labeling double-stranded DNA with 1 μM of SYTOX. The merged images are presented in (f) (Sharma et al, 2008).