(a) The training protocol for experiment 3, with each arrow indicating 1 day, unless otherwise indicated. Mean (±SEM) percentage of CS-elicited freezing is shown for each group at training (b), extinction (c), and test (d) in experiment 3. Immediate injection of DCS resulted in significantly less freezing at test compared with the saline control group in adolescent rats. However, a 4 h delayed injection of DCS resulted in no difference compared with the saline control group.