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. 2009 Nov 18;35(3):792–805. doi: 10.1038/npp.2009.188

Figure 1.

Figure 1

Screen of psychoactive drugs for their global effects on histone acetylation. Cortical (CTX; a, b) and hippocampal astrocytes (HPC; c, d) were treated with VPA (1 and 10 mM), LTG (10 and 100 μM), CBZ (10 and 100 μM), VEN (1 and 10 μM), CIT (1 and 10 μM), AMI (1 and 10 μM), or with the HDAC inhibitor TSA (0.2 μM) for 24 h. After drug treatment, cell lysates were prepared and subjected to western blot analysis using polyclonal antibodies against AcH3K9 and AcH4. AcH3K9 and AcH4 band intensities were normalized to α-actin. The bars are depicted as the percentage of optical band densities (mean±SE, n=2) calculated relative to the value that corresponds to histone acetylation induced by TSA (set to 100%); t-test: *p<0.05 vs untreated cells, **p<0.005 vs untreated cells.