Skip to main content
. 2009 Nov 18;35(3):792–805. doi: 10.1038/npp.2009.188

Figure 2.

Figure 2

VPA induces dynamic changes of AcH3K9 and AcH4 patterns in astrocytes from different brain regions. Cortical (a, b) and hippocampal (c, d) astrocytes were exposed to 1 and 10 mM VPA for 24, 72, and 72 h, followed by a 48 h washout period, whereas a single dose of TSA (0.2 μM) was applied for 24 and 72 h. The level of AcH3K9 and AcH4 was monitored by western blot analysis and detected with AcH3K9- and AcH4-specific antibodies. α-Actin immunoreactivity served as a control for protein loading. Optical densities of AcH3K9- and AcH4-specific bands were normalized to α-actin. Data represent mean±SE (n=2) of the percentage of optical band densities calculated relative to the value that corresponds to histone acetylation induced by 24 h treatment with 0.2 μM TSA (set to 100%); t-test: *p<0.05 vs untreated cells, **p<0.005 vs untreated cells, #p<0.05 vs 1 mM VPA depletion treatment, §p<0.05 vs 10 mM VPA depletion treatment.