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. 2009 Nov 18;35(3):792–805. doi: 10.1038/npp.2009.188

Figure 6.

Figure 6

Differential effects of VPA and AMI on H3K9 dimethylation levels. Cortical (CTX) and hippocampal (HPC) astrocytes were treated with VPA (1 and 10 mM), AMI (1 and 10 μM) and NaB (0.1 and 1 mM) for 24 h. Western blot analysis was performed using antibodies directed against (a, b) dimethyl-H3K9 (α-2MeH3K9) or (c, d) dimethyl-H3K27 (α-2MeH3K9). As a control for protein loading, α-actin-specific antibody was used. Data represent mean±SE (n=3) of the percentage of optical band densities normalized to α-actin immunoreactivity. Control is set to 1; t-test: *p<0.05.