Fig. 4. Met4p is stabilized in the presence of a cim3 mutation. (A) The stability of HA-Met4p expressed in cim3-1 mutant cells grown in repressive and non-repressive conditions was analyzed as described in Figure 3B. (B) Repression of the MET25 gene was monitored in cim3-1 and cim5-1 mutant cells. Cells were grown and RNA extracted as described in Figure 1. The wild-type (wt) strain used in this assay was the YPH499 strain, which was congenic to both mutant strains.