Figure 4.

Fluorescence microscopy of cultures obtained from wound digests. (A) Murine cells derived from digestion of control defects, no presence of PKH67. (B) Populations of PKH-67-stained HUCPVCs were identified alongside murine-derived cells following digestion of treatment defects. Intact DAPI-stained nuclei indicate viable cells. (C) Immunocytochemical analysis for Ki-67 (red, visualized using Texas red) colocalized to DAPI-stained cell nuclei of PKH67-positive (green) HUCPVCs. Field widths: (A and B) 29 µm, (C) 214 µm.