Figure 7.

Saturation analysis of specific [³H]DPCPX binding (0.1–10 nM) to cortico-hippocampal membranes (40 μg protein) from chronic caffeine- (3 mg/kg/day, for 22 days, ♦) and vehicle- (○) treated mice. Inset: B_max obtained from nonlinear regression analysis. Nonspecific binding was determined at all [³H]DPCPX concentrations by the addition of 2 μM XAC. All points represent mean±standard error of mean (SEM) of n=6, and each saturation experiment was performed in duplicate. ^*p<0.05, vs control, calculated using the extra sum-of-squares F test.