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Colocalization of pERK1/2 and Fos (left panel) or pCREB (right panel). These rats were injected with control vector T0Z before the morphine withdrawal protocol. Double-label immunostaining showed that pERK1/2 was colocalized with either Fos or pCREB immunostaining. Arrows indicate double-labeled cells. Scale bar, 100 μm.
