Table V. Influence of Hsp104p overexpression on the stability of different [PSI+PS] strains.
[PSI+] strain | Plasmid | Number of transformants |
||
---|---|---|---|---|
white or pink | with red sectors | red | ||
#1 | pFL44-HSP104 | 62 (96.9%) | 2 (3.1%) | 0 |
pEMBLyex4 | 140 (97.9%) | 0 | 3 (2.1%) | |
#2 | pFL44-HSP104 | 243 (97.9%) | 5 (2.1%) | 0 |
pEMBLyex4 | 390 (100%) | 0 | 0 | |
#3 | pFL44-HSP104 | 196 (78.7%) | 11 (4.4%) | 42 (16.9%) |
pEMBLyex4 | 135 (88.2%) | 3 (2.0%) | 15 (9.8%) | |
#4 | pFL44-HSP104 | 34 (49.3%) | 32 (46.3%) | 3 (4.4%) |
pEMBLyex4 | 89 (98.9%) | 0 | 1 (1.1%) |
The pEMBLyex4 vector lacking the HSP104 gene was used as a control. The data represent an average from three independent transformants. The percentage of transformants of each type is indicated in parentheses.