Fig. 6. Effect of C–terminal JLN mutation on assembly of KvLQT1 C–terminus. (A) Diagram illustrating protein constructs tagged with Xpress (xC.T.354–676) or with myc (mycJLN). Full-length KvLQT1 protein is shown on top as in Figure 4A. The deletion–insertion mutation at KvLQT1 residue 544 (see Figure 2) is indicated by hatching. (B) 35S–labelled Xpress-tagged KvLQT1 and myc-tagged JLN C–termini were obtained by in vitro translation (Input). They were precipitated either with anti-Xpress antibodies (anti-Xpress) or with anti-myc antibodies (anti-myc). Proteins were separated by SDS–PAGE followed by autoradiography. Molecular weight markers are on the left. Lanes 1, 4 and 9 contained only xC.T.354–676, lanes 2, 6 and 7 only mycJLN, and lanes 3, 5 and 8 xC.T.354–676 and mycJLN as starting material.