Table 2.
QTL detected and respective statistics for body composition traits and food consumption
| Trait | Nearest Marker | MMU | Peak Position, Mb | Naive LOD | GRAIP LOD | CI, Mb | % Var | Additive ± SE | Dominance ± SE |
|---|---|---|---|---|---|---|---|---|---|
| ∼4 wk of age | |||||||||
| Body mass, g | JAX00005495 | 1 | 77 | 7.7 | 3.7 | 74–82 | 1.9 | 0.54 ± 0.15 | 0.41 ± 0.21 |
| JAX00155508 | 7 | 109 | 8.0 | 4.2* | 95–113 | 3.2 | 0.59 ± 0.14† | 0.49 ± 0.21 | |
| % Fat | JAX00005495 | 1 | 77 | 17.5 | 4.7* | 75–79‡ | 6.7 | 1.30 ± 0.17† | 0.25 ± 0.25 |
| JAX00010715 | 1 | 148 | 16.3 | 4.7* | 144–150‡ | 5.6 | 1.06 ± 0.16† | −0.52 ± 0.25 | |
| JAX00107680 | 3 | 56 | 9.5 | 4.7* | 40–59 | 5.9 | −1.22 ± 0.17† | −0.06 ± 0.26 | |
| JAX00154099 | 7 | 90 | 7.9 | 3.6 | 75–99 | 2.7 | −0.41 ± 0.17 | 1.03 ± 0.26 | |
| JAX00190351 | 8 | 89 | 20.4 | 4.7* | 82–93‡ | 8.8 | 1.39 ± 0.17† | −0.81 ± 0.24 | |
| JAX00033353 | 12 | 12 | 10.4 | 4.2* | −22 | 3.7 | −0.96 ± 0.17† | 0.16 ± 0.25 | |
| % Lean | JAX00005495 | 1 | 77 | 12.1 | 4.7* | 74–91 | 5.2 | −1.24 ± 0.19† | −0.34 ± 0.27 |
| JAX00010715 | 1 | 148 | 16.1 | 4.7* | 134–172 | 6.0 | −1.12 ± 0.17† | −0.80 ± 0.27 | |
| JAX00107680 | 3 | 56 | 8.1 | 4.0* | 39–59 | 6.8 | 1.37 ± 0.19† | −0.17 ± 0.27 | |
| JAX00675742 | 8 | 92 | 22.0 | 4.7* | 83–95‡ | 10.0 | −1.66 ± 0.18† | 0.66 ± 0.26 | |
| ∼6 wk of age | |||||||||
| Body mass, g | JAX00139789 | 6 | 36 | 10.2 | 4.1* | 25–43 | 1.5 | 0.63 ± 0.18 | 0.004 ± 0.278 |
| % Fat | JAX00005495 | 1 | 77 | 6.9 | 3.9* | 74–89 | 3.4 | 1.12 ± 0.22† | 0.09 ± 0.31 |
| JAX00010715 | 1 | 149 | 7.6 | 4.5* | 146–152 | 3.2 | 1.01 ± 0.20† | −0.27 ± 0.31 | |
| JAX00675742 | 8 | 92 | 15.5 | 4.7* | 86–94‡ | 8.9 | 1.78 ± 0.21† | −0.68 ± 0.31 | |
| % Lean | JAX00010715 | 1 | 149 | 6.2 | 3.5 | 143–158 | 2.6 | −0.85 ± 0.20 | 0.59 ± 0.32 |
| JAX00675742 | 8 | 92 | 12.3 | 4.7* | 68–99 | 8.3 | −1.76 ± 0.21† | 0.48 ± 0.31 | |
| JAX00391461 | 14 | 120 | 5.3 | 3.5 | 109– | 3.0 | 1.07 ± 0.22† | −0.53 ± 0.31 | |
| ∼8 wk of age | |||||||||
| Body mass, g | JAX00263199 | 1 | 116 | 6.9 | 3.5 | 95–141 | 1.1 | 0.65 ± 0.22 | 0.37 ± 0.33 |
| JAX00127022 | 5 | 11 | 9.4 | 4.7* | −16 | 1.8 | −0.62 ± 0.24 | −0.37 ± 0.33 | |
| JAX00139789 | 6 | 36 | 10.6 | 4.7* | 25–40 | 1.0 | 0.63 ± 0.22 | −0.08 ± 0.33 | |
| JAX00415862 | 16 | 24 | 7.3 | 3.5 | 11–28 | 1.5 | −0.73 ± 0.23 | 0.33 ± 0.33 | |
| % Fat | JAX00160567 | 8 | 37 | 10.7 | 4.7* | 21–52 | 6.1 | 1.56 ± 0.22† | −0.17 ± 0.30 |
| JAX00695061 | 9 | 57 | 6.0 | 3.6 | 50–70 | 2.1 | 0.90 ± 0.22 | 0.38 ± 0.32 | |
| % Lean | JAX00160567 | 8 | 37 | 9.9 | 4.7* | 27–49 | 6.3 | −1.54 ± 0.21† | −0.04 ± 0.30 |
| JAX00190133 | 7 | 80 | 5.9 | 3.6 | 74–87 | 5.2 | 0.75 ± 0.21 | −1.7 ± 0.30† | |
| Post exercise | |||||||||
| Body mass, g | JAX00247128 | 1 | 39 | 8.6 | 4.6* | 35–42 | 1.3 | 0.48 ± 0.20 | −0.60 ± 0.28 |
| JAX00261568 | 1 | 106 | 8.4 | 4.2* | 89–141 | 1.0 | 0.52 ± 0.19 | 0.21 ± 0.28 | |
| JAX00127022 | 5 | 1 | 9.5 | 4.4* | −19 | 0.8 | −0.46 ± 0.20 | −0.23 ± 0.28 | |
| JAX00139789 | 6 | 36 | 8.1 | 3.5 | 24–41 | 0.5 | 0.37 ± 0.19 | 0.02 ± 0.28 | |
| % Fat | JAX00011133 | 1 | 155 | 11.8 | 4.7* | 130–183 | 4.7 | 0.90 ± 0.15† | −0.08 ± 0.21 |
| JAX00105078 | 3 | 21 | 6.8 | 3.7 | 15–25 | 3.6 | −0.09 ± 0.14 | 1.13 ± 0.21† | |
| JAX00131820 | 5 | 77 | 8.0 | 4.5* | 73–91 | 3.7 | −0.82 ± 0.15† | −0.21 ± 0.21 | |
| JAX00190133 | 7 | 80 | 7.2 | 4.4* | 77–83 | 5.6 | −0.56 ± 0.15 | 1.23 ± 0.21† | |
| JAX00675742 | 8 | 93 | 10.4 | 4.7* | 64–96 | 6.6 | 1.06 ± 0.15† | −0.35 ± 0.21 | |
| JAX00391461 | 14 | 120 | 7.7 | 4.7* | 78– | 3.8 | −0.82 ± 0.15† | 0.37 ± 0.21 | |
| % Lean | JAX00268776 | 1 | 140 | 10.3 | 4.7* | 131–170 | 4.3 | −0.90 ± 0.16† | 0.16 ± 0.23 |
| JAX00131820 | 5 | 77 | 9.0 | 4.7* | 67–92 | 5.4 | 1.02 ± 0.16† | 0.47 ± 0.22 | |
| JAX00190133 | 7 | 80 | 7.2 | 4.7* | 77–82 | 6.5 | 0.64 ± 0.16 | −1.43 ± 0.22† | |
| JAX00675742 | 8 | 93 | 12.4 | 4.7* | 65–97 | 8.6 | −1.31 ± 0.15† | 0.41 ± 0.23 | |
| JAX00391461 | 14 | 120 | 7.4 | 4.7* | 104– | 4.4 | 0.96 ± 0.16† | −0.30 ± 0.23 | |
| % Change in body mass | JAX00031382 | 11 | 105 | 4.6 | 3.5 | 102–110 | 2.3 | −1.17 ± 0.27† | −0.06 ± 0.37 |
| % Change in % fat | JAX00277411 | 1 | 178 | 5.5 | 3.8 | 170–183 | 3.2 | 4.56 ± 0.93† | 2.03 ± 1.36 |
| JAX00132785 | 5 | 90 | 8.5 | 4.7* | 48–100 | 4.1 | −5.35 ± 0.96† | −2.27 ± 1.34 | |
| % Change in % lean | JAX00133006 | 5 | 93 | 6.0 | 4.1* | 71–103 | 3.5 | 1.47 ± 0.27† | 0.20 ± 0.37 |
| Food intake, g | JAX00155961 | 7 | 115 | 10.8 | 4.7 | 112–117‡ | 7.8 | 1.4 ± 0.17† | 0.002 ± 0.25 |
| Food intake/mass | JAX00612506 | 6 | 67 | 6.4 | 4.6* | 59–73 | 4.0 | −0.04 ± 0.01† | −0.05 ± 0.01 |
| JAX00645408 | 7 | 83 | 6.7 | 4.2* | 78–92 | 3.4 | 0.03 ± 0.01 | −0.05 ± 0.01† | |
| Energy balance | JAX00160567 | 8 | 37 | 5.4 | 4.1* | 31–44 | 4.4 | −5.94 ± 1.12† | −0.33 ± 1.57 |
Beginning at 8 wk of age body composition measures were taken immediately before (Pre) and after (Post) 6 days of wheel access. Food consumption was quantified as the amount eaten over the entire 6-day access to running wheels, and values are presented per gram of body mass [(Pre wheel access + Post wheel access)/2]. Percent body fat (and lean) was calculated as (fat mass/body mass) × 100. Percent change variables were calculated as [(Post − Pre)/Pre] × 100. Logarithm of odds (LOD) exceeding the 95% (P ≤ 0.05, LOD ≥3.9) permutation threshold are denoted by
; other quantitative trait loci (QTL) exceeded the 90% (P ≤ 0.1, LOD ≥3.5) threshold. Confidence intervals (CIs) for QTL positions were obtained with a 1.0-LOD drop in Mb. CIs are relative to the Genome Reshuffling for Advanced Intercross Permutation (GRAIP)-permuted LOD score with the exception of those denoted by
, which are relative to the naive LOD score. Percentage of phenotypic variance accounted for by the QTL effect: for additive and dominance effects, positive values indicate increasing effect of the high runner (HR) allele or increasing effect of the heterozygote, respectively.
Additive and dominance effects were statistically significant at P < 0.05. Body mass QTL at ∼8 wk of age have been previously published in Kelly et al. (21) and are simply reproduced here for completeness.