Fig. 3.
Mist1−/− mice display normal plasma cell development and antibody secretion. A: blood serum and fecal pellets from wild-type and Mist1−/− mice were assayed for IgA levels by ELISA. Each data point represents an individual mouse (n = 8 mice/group). Mice were matched by age and sex between wild-type and Mist1−/− populations. B: fractional representation of B cell subsets in wild-type and Mist1−/− spleens as determined by flow cytometry as follows: follicular B cells (IgDhi, IgMlo), marginal zone (MZ) B cells (IgDlo, IgMhi), B-1a B cells (CD5hi, IgM+), B-1b B cells (CD5lo, B220lo), B-2 B cells (CD23+, IgM+), and plasma cells (CD138+, B220+). C: flow cytometric analysis of bone marrow harvested from wild-type (left) and Mist1−/− (right) femurs. CD138+, B220+ plasma cells are shown in boxes (n = 4 mice/group).