Intimate association of LYVE-1+ and CD31+ cells in the HVS. (A–C) Representative cross-sectional micrographs demonstrating the intimate link between LYVE-1+ and CD31+ cells at both embryonic (A) and postnatal (B) stages. (C) Higher magnification views of the boxed areas in (A) and (B), respectively. LYVE-1+ cells appeared abutting the CD31+ cells (C, left) or linearly arranged along the CD31+ branches (C, right). Red: LYVE-1; green: CD31; blue: DAPI nuclei staining. L, lens; V, vitreous; R, retina. Scale bars: 100 μm (A, B); 25 μm (C).